Detects mouse Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 5% cross‑reactivity with rmIntegrin beta 1, rmIntegrin beta 4, and rmIntegrin beta 7 is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 2 Gln24-Asn702 Accession # P11835
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 2/CD18 in Mouse Lymph Node Cells by Flow Cytometry. Mouse lymph node cells were stained with Goat Anti-Mouse Integrin beta 2/CD18 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2618F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 2/CD18
Integrin beta 2 is a 90-100 kDa type I transmembrane protein that belongs to Group A of the Integrin beta subunit family of molecules. It is expressed by multiple hematopoietic cells, including basophils, neutrophils, NK cells, monocytes, eosinophils, B cells, mast cells, and and macrophages, as well as endothelial cells. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking. Over amino acids (aa) 24-702, mouse Integrin beta 2 shares 90% and 81% aa sequence identity with rat and human Integrin beta 2, respectively.
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