Mouse Integrin  beta 2/CD18 Fluorescein-conjugated Antibody

FAB2618F has been discontinued. View all Integrin beta 2/CD18 products.
  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
FAQs
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 5% cross‑reactivity with rmIntegrin beta 1, rmIntegrin beta 4, and rmIntegrin beta 7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 2
    Gln24-Asn702
    Accession # P11835
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Integrin  beta 2/CD18 in Mouse Lymph Node Cells by Flow Cytometry. Mouse lymph node cells were stained with Goat Anti-Mouse Integrin  beta 2/CD18 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2618F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 2/CD18
Integrin beta 2 is a 90-100 kDa type I  transmembrane protein that belongs to Group A of the Integrin beta subunit family of molecules. It is expressed by multiple hematopoietic cells, including basophils, neutrophils, NK cells, monocytes, eosinophils, B cells, mast cells, and and macrophages, as well as endothelial cells. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking. Over amino acids (aa) 24-702, mouse Integrin beta 2 shares 90% and 81% aa sequence identity with rat and human Integrin beta 2, respectively.
  • Entrez Gene IDs:
    3689 (Human); 16414 (Mouse); 309684 (Rat)
  • Alternate Names:
    959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression.
    Authors: Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T
    J Immunol, 2014;192(1):407-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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