|Detection of Jagged 2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Jagged 2 APC‑conjugated Monoclonal Antibody (Catalog # FAB4748A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.|
Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. This family is known to regulate cell fate determination during development (2-6). Jagged 2 is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a 1059 aa extracellular region, a 21 aa transmembrane region, and a 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), and a cysteine-rich region just proximal to the transmembrane segment (3). Over aa 27-1084, mouse Jagged 2 shares 97% and 91% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). Jagged 2 is one of five ligand for Notch in mammals. Its binding affinity is predicted on the degree of Notch glycosylation (7). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, gamma δ T cell lineage differentiation, and the establishment of functional natural killer cell lines (4-6).