Mouse Jagged 2 PE-conjugated Antibody

FAB4748P has been discontinued. View all Jagged 2 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects mouse Jagged 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Jagged 1, rhJagged 2, recombinant rat Jagged 1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 746513
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Jagged 2
    Accession # Q9QYE5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Jagged 2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Jagged 2 PE‑conjugated Monoclonal Antibody (Catalog # FAB4748P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Jagged 2

Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. This family is known to regulate cell fate determination during development (2-6). Jagged 2 is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a 1059 aa extracellular region, a 21 aa transmembrane region, and a 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), and a cysteine-rich region just proximal to the transmembrane segment (3). Over aa 27-1084, mouse Jagged 2 shares 97% and 91% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). Jagged 2 is one of five ligand for Notch in mammals. Its binding affinity is predicted on the degree of Notch glycosylation (7). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems,  gamma δ T cell lineage differentiation, and the establishment of functional natural killer cell lines (4-6).

  • References:
    1. Tsai, S. et al. (2000) Blood, 96:950.
    2. Shawber, C. et al. (1996) Dev. Biol. 180:370.
    3. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
    4. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
    5. DeHart, S. et al. (2005) Blood 105:3521.
    6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
    7. Takeuchi, H. and R.S. Haltiwanger (2014) Biochem. Biophys. Res. Commun. 453:235.
  • Entrez Gene IDs:
    3714 (Human); 16450 (Mouse); 29147 (Rat)
  • Alternate Names:
    HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2
Related Research Areas


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