Mouse KLRG1 APC-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB6944A-025
Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry.
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Mouse KLRG1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse KLRG1 in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1151A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived mouse KLRG1
Glu57-Tyr188
Accession # O88713
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE‑conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Rabbit Anti-Mouse KLRG1 APC‑conjugated Monoclonal Antibody (Catalog # FAB6944A) or (B) Normal Rabbit IgG Allophycocyanin Control (Catalog # IC105A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLRG1

KLRG1 (Killer cell Lectin-like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C-type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C-type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30-40 kDa N-glycosylated molecule that forms disulfide-linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6-8). KLRG1 is expressed on T cells found in cord blood, but it is down-regulated postnatally and is subsequently re-expressed on antigen-exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus-specific CD8+ T cells during chronic virus infection (10-12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM-dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E-Cadherin on dendritic cells (DC) can induce an anti-inflammatory DC phenotype (increased IL-10 production and decreased IL-6 and TNF-alpha production) (15).

References
  1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
  2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
  3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
  4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
  5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
  6. Voehringer, D. et al. (2002) Blood 100:3698.
  7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
  8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
  9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
  10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
  11. Thimme, R. et al. (2005) J. Virol. 79:12112.
  12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
  13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
  14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
  15. Banh, C. et al. (2009) Blood 114:5299.
Long Name
Killer Cell Lectin-like Receptor Subfamily G Member 1
Entrez Gene IDs
10219 (Human); 50928 (Mouse); 58975 (Rat)
Alternate Names
2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600

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