Mouse L1CAM APC-conjugated Antibody

FAB5674A has been discontinued. View all L1CAM products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm)MAdCAM-1, or rmOCAM is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 555
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse cerebellum-derived partially purified L1CAM
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of L1CAM in Mouse splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse L1CAM APC‑conjugated Monoclonal Antibody (Catalog # FAB5674A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: L1CAM
L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). L1CAM is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM (2). Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail (3). Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins (4). L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub (4). The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis (1). Overexpression promotes tumor cell invasion and motility, growth in nude mice, and tumor metastasis (1). Proteolytic processing by ADAM10 and presenilin/ gamma -secretase is essential for the nuclear signaling of L1CAM in human carcinoma cell lines (1). Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome (5, 6).
  • References:
    1. Riedle, S. et al. (2009) Biochem. J. 420:391.
    2. Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
    3. Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
    4. Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
    5. Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
    6. Striha, L. et al. (2000) J. Child Neurol. 15:239.
  • Long Name:
    Cell Adhesion Molecule L1
  • Entrez Gene IDs:
    3897 (Human); 16728 (Mouse); 50687 (Rat)
  • Alternate Names:
    antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; L1CAM; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1
Related Research Areas


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