Mouse Leptin/OB Antibody

Catalog # Availability Size / Price Qty
AF498
AF498-SP
Mouse Leptin/OB Antibody in Data
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Product Details
Citations (13)
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Mouse Leptin/OB Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin/OB in ELISAs and Western blots. In sandwich ELISAs, approximately 40% cross-reactivity with recombinant rat Leptin/OB is observed and less than 0.2% cross-reactivity with recombinant human Leptin/OB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Leptin/OB
Val22-Cys167
Accession # Q544U0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin/OB (Catalog # 498-OB)
Immunohistochemistry
5-15 µg/mL
See below

Mouse Leptin/OB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Leptin/OB Biotinylated Antibody (Catalog # BAF498)

Standard: Recombinant Mouse Leptin Protein, CF (Catalog # 498-OB)

Neutralization
Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.05-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Leptin/OB antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Leptin/OB in Mouse Brain. Leptin/OB was detected in perfusion fixed frozen sections of mouse brain using Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Product Datasheets

Citations for Mouse Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Localized Antileptin Therapy Prevents Aortic Root Dilatation and Preserves Left Ventricular Systolic Function in a Murine Model of Marfan Syndrome
    Authors: S Fisch, N Bachner-Hi, O Ertracht, L Guo, Y Arad, D Ben-Zvi, R Liao, J Schneiderm
    J Am Heart Assoc, 2020;9(10):e014761.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
    Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
    Oncogene, 2019;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary gland
    Authors: I Tenvooren, MZ Jenks, H Rashid, KL Cook, JK Muhlemann, C Sistrunk, J Holmes, K Wang, K Bonin, K Hodges, HW Lo, A Shaikh, IG Camarillo, SA Lelièvre, V Seewaldt, PA Vidi
    Oncogene, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells
    Authors: V Frodermann, D Rohde, G Courties, N Severe, MJ Schloss, H Amatullah, CS McAlpine, S Cremer, FF Hoyer, F Ji, ID van Koever, F Herisson, L Honold, GS Masson, S Zhang, J Grune, Y Iwamoto, SP Schmidt, GR Wojtkiewic, IH Lee, K Gustafsson, G Pasterkamp, SCA de Jager, RI Sadreyev, J MacFadyen, P Libby, P Ridker, DT Scadden, K Naxerova, KL Jeffrey, FK Swirski, M Nahrendorf
    Nat. Med., 2019;25(11):1761-1771.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
    Authors: Q Zeng, X Luo, M Han, W Liu, H Li
    EBioMedicine, 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain
    Authors: C Nozaki, E Nent, A Bilkei-Gor, A Zimmer
    Sci Rep, 2018;8(1):10827.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Leptin sustains spontaneous remyelination in the adult central nervous system
    Authors: K Matoba, R Muramatsu, T Yamashita
    Sci Rep, 2017;7(0):40397.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion
    Authors: KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang
    Data Brief, 2017;15(0):567-572.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
    Authors: Kim, Min Soo, Yan, Jingqi, Wu, Wenhe, Zhang, Guo, Zhang, Yalin, Cai, Dongshen
    Nat Immunol, 2015;16(5):525-33.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation.
    Authors: Schroeter M, Eschholz N, Herzberg S, Jerchel I, Leifheit-Nestler M, Czepluch F, Chalikias G, Konstantinides S, Schafer K
    Arterioscler Thromb Vasc Biol, 2013;33(5):980-7.
  11. Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brain.
    Authors: Balland E, Dam J, Langlet F, Caron E, Steculorum S, Messina A, Rasika S, Falluel-Morel A, Anouar Y, Dehouck B, Trinquet E, Jockers R, Bouret S, Prevot V
    Cell Metab, 2013;19(2):293-301.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Leptin derived from adipocytes in injured peripheral nerves facilitates development of neuropathic pain via macrophage stimulation.
    Authors: Maeda T, Kiguchi N, Kobayashi Y, Ikuta T, Ozaki M, Kishioka S
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(31):13076-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002;13(2):225-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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