Mouse M-CSF R PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse M-CSF R in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human M-CSF R, recombinant mouse (rm) PDGF R beta, or rmFlt-3 Ligand is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 460615
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R
    Accession # P09581
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of M‑CSF R in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse M-CSF R PerCP‑conjugated Monoclonal Antibody (Catalog # FAB3818C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares > 99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3 Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and dendritic cells (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

  • References:
    1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
    2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
    3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
    4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
    5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
    6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
    7. Dai, X. et al. (2002) Blood 99:111.
    8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
    9. Li, M. et al. (2004) J. Neurochem. 91:623.
    10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
  • Long Name:
    Macrophage Colony Stimulating Factor Receptor
  • Entrez Gene IDs:
    1436 (Human)
  • Alternate Names:
    CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; MCSFR; M-CSFR; Proto-oncogene c-Fms
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PerCP-conjugated Isotype Control

Ctrl IC013C
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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