Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 191004
View all NKG2D/CD314 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NKG2D/CD314
Phe94-Val232
Accession # Q4FJM0

Specificity

Detects mouse NKG2D/CD314 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse NKG2D/CD314 Biotinylated Antibody

NKG2D/CD314 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

NKG2D/CD314 in Mouse Splenocytes.

NKG2D/CD314 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse NKG2D/CD314 Biotinylated Monoclonal Antibody (Catalog # BAM1547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse NKG2D/CD314 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed enriched human NK cells and mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse NKG2D/CD314 Fc Chimera (Catalog # 139-NK) under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 4.5 using BAM1547 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, delta, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

References

  1. Bauer, S. et al. (1999) Science 285:727.
  2. Wu, J. et al. (1999) Science 285:730.
  3. Diefenbach, A. et al. (2001) Nature 413:165.
  4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
  5. NKG2D and its Ligands; www.RnDSystems.com.

Long Name

Natural Killer G2D

Alternate Names

CD314, D12S2489E, KLRK1

Entrez Gene IDs

22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)

Gene Symbol

KLRK1

UniProt

Q4FJM0

Additional NKG2D/CD314 Products

Product Documents for Mouse NKG2D/CD314 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse NKG2D/CD314 Biotinylated Antibody

For research use only

Citations for Mouse NKG2D/CD314 Biotinylated Antibody

Customer Reviews for Mouse NKG2D/CD314 Biotinylated Antibody (2)

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  • Mouse NKG2D/CD314 Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 02/04/2022
    Mouse NKG2D/CD314 Biotinylated Antibody BAM1547
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22942430
    Species: Mouse
    Verified Customer | Posted 01/15/2015

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Protocols

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