Mouse NKG2D/CD314 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1547
NKG2D/CD314 in Mouse Splenocytes.
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Product Details
Citations (2)
FAQs
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Reviews (1)

Mouse NKG2D/CD314 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NKG2D/CD314 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 191004
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKG2D/CD314
Phe94-Val232
Accession # Q4FJM0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse NKG2D/CD314 Fc Chimera (Catalog # 139-NK) under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry NKG2D/CD314 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

NKG2D/CD314 in Mouse Splenocytes. NKG2D/CD314 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse NKG2D/CD314 Biotinylated Monoclonal Antibody (Catalog # BAM1547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

References
  1. Bauer, S. et al. (1999) Science 285:727.
  2. Wu, J. et al. (1999) Science 285:730.
  3. Diefenbach, A. et al. (2001) Nature 413:165.
  4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
  5. NKG2D and its Ligands; www.RnDSystems.com.
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

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Citations for Mouse NKG2D/CD314 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2012-09-27;133(2):334-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. NKG2D blockade prevents autoimmune diabetes in NOD mice.
    Authors: Ogasawara K, Hamerman JA, Ehrlich LR, Bour-Jordan H, Santamaria P, Bluestone JA, Lanier LL
    Immunity, 2004-06-01;20(6):757-67.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC

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Mouse NKG2D/CD314 Biotinylated Antibody
By Anonymous on 02/04/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Splenocytes Species: Mouse