Mouse Notch-1 PerCP-conjugated Antibody

IC5267C has been discontinued. View all Notch-1 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Notch-1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # N1A
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse embryonic fibroblast cell line PA317-derived recombinant mouse Notch-1
    Accession # Q01705
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Notch‑1 in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Mouse Notch‑1 PerCP‑conjugated Monoclonal Antibody (Catalog # IC5267C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram).  View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Notch-1

Notch-1 is a 210-240 kDa type I transmembrane glycoprotein that is one of four Notch homologues involved in developmental processes (1-3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2-4). Notch can specify binary cell fates. For example, it promotes T cell over B cell development from a common precursor (2). Mouse Notch-1 is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1707 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/notch repeats (LNR), a 21 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain that contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11th and 12th EGF-like repeats, that bind ligands such as Jagged-1 and -2, plus Delta-like-1, -3, and -4 plus DNER in humans, correspond to aa 412-488 in mouse Notch-1 (5, 6). Within the 36 EGF-like repeats, N-linked and multiple O-linked glycans are known to exist. These include O-fucose,O-glucose, O-GlcNAc and O-xylose based glycans. Although the best studied modification involves fucose, the mammalian system is very complex and unlikely fully explains the facilitation of delta-like signaling with an inhibition of Jagged-induced signaling. O-linked glucose appears to be essential for Notch activation, while O-GlcNAc modifications have unclear functions (7). The Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, generating a heterodimer through the interaction of a hydrophobic area C-terminal to the LNR on the extracellular region with the transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (9, 10). Mouse Notch-1 ECD aa 19-526, which includes the first 13 EGF repeats, shows 94%, 91%, 86% and 79% aa sequence identity with corresponding regions of rat, human, canine, and chicken Notch-1, respectively. This region also exhibits 55-58% aa sequence identity with human Notch-2 and Notch-3.

  • References:
    1. Ellisen, L.W. et al. (1991) Cell 66:649.
    2. de LaCoste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
    3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
    4. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.
    5. Chillakuri, C. et al. (2012) Semin. Cell Dev. Biol. 23:421.
    6. Hambleton, S. et al. (2004) Structure 12:2173.
    7. Takeuchi, H. and R.S. Haltiwanger (2014) Biochem. Biophys. Res. Commun. 453:235.
    8. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
    9. Fiuza, U-M and A.M. Arias (2007) J. Endocrinol. 194:459.
    10. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
  • Entrez Gene IDs:
    4851 (Human); 25496 (Rat)
  • Alternate Names:
    EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
Related Research Areas

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