Mouse Notch-2 PE-conjugated Antibody

FAB5196P has been discontinued. View all Notch-2 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity with rhNotch-1, -3, recombinant mouse (rm) Notch-1, rmNotch-3, or rrDLL1 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 605724
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2
    Leu26-Val528 (predicted)
    Accession # O35516
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Notch‑2 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Notch‑2 PE‑conjugated Monoclonal Antibody (Catalog # FAB5196P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Notch-2

Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins.  It is found on multiple cell types and exhibits multiple functions.  On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation.  Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second of which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor.  There are three potential splice variants.  One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713.  Over aa 26-528, mouse Notch-2 shares 96% and 94% aa sequence identity with rat and human Notch-2, respectively.   

  • Entrez Gene IDs:
    4853 (Human); 29492 (Rat)
  • Alternate Names:
    AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2
Related Research Areas

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