Mouse OX40 Ligand/TNFSF4 Fluorescein-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse OX40 Ligand/TNFSF4 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) OX40 Ligand, rhLIGHT, recombinant mouse (rm) Fas Ligand and less than 1% cross-reactivity with rmTNF‑ alpha, rhGITR Ligand, rhTRANCE, and rhVEGI is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4
    Accession # P43488
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of OX40 Ligand/TNFSF4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS and Recombinant Mouse GM-CSF (Catalog # 415-ML) were stained with Goat Anti-Mouse OX40 Ligand/TNFSF4 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1236F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) residue protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and murine OX40L share 46% sequence identity at the amino acid level (1). The OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) that is expressed predominantly on activated CD4+ T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is up-regulated by CD40 ligation (3). Engagement of the OX40-OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40-OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40-OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease, and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).

  • References:
    1. Baum, P.R. et al. (1994) EMBO J. 13:3992.
    2. Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
    3. Stuber, E. et al. (1995) Immunity 2:507.
    4. Malstrom, V. et al. (2001) J. Immunol. 166:6972.
    5. Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
    6. Weinberg, A.D. (2002) Trends Immunol. 23:102.
  • Entrez Gene IDs:
    7292 (Human); 22164 (Mouse); 89814 (Rat)
  • Alternate Names:
    CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Fluorescein-conjugated Control

Ctrl IC108F 1
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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