Mouse p53 Antibody Summary
Accession # NP_035770
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Immunoprecipitation of Mouse p53.
Balb/3T3 mouse embryonic fibroblast cell line was untreated
(-) or treated (+) with 1 μM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 μg of cell lysate following incubation with 1‑3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
Citations for Mouse p53 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Early-life nutrition influences thymic growth in male mice that may be related to the regulation of longevity.
Authors: Chen JH, Tarry-Adkins JL, Heppolette CA, Palmer DB, Ozanne SE
Clin. Sci., 2010;118(6):429-38.
Sample Types: Tissue Homogenates
Applications: Western Blot
Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
Authors: Chen JH, Jones RH, Tarry-Adkins J, Smith NH, Ozanne SE
Exp. Cell Res., 2008;314(16):3075-80.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
ExactaChIP Chromatin Immunoprecipitation Kits
Reviews for Mouse p53 Antibody
There are currently no reviews for this product. Be the first to review Mouse p53 Antibody and earn rewards!
Have you used Mouse p53 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image