Mouse PD-1 Antibody

Catalog # Availability Size / Price Qty
MAB77381-SP
MAB77381-100
Detection  of mouse PD‑1 by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Mouse PD-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PD-1 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 996221
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line, NS0-derived mouse PD-1
Met1-Gln167
Accession # Q02242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of mouse PD‑1 by Western Blot. View Larger

Detection of mouse PD‑1 by Western Blot. Western blot shows lysates of EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse PD‑1 Monoclonal Antibody (Catalog # MAB77381) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for PD‑1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PD-1 in HEK293 Human Cell Line Transfected with  Mouse PD-1 and eGFP by Flow Cytometry. View Larger

Detection of PD-1 in HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-1 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse PD-1 Monoclonal Antibody (Catalog # MAB77381) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  5. Latchman Y. et al. (2001) Nat. Immun. 2:261.
  6. Tamura, H. et al. (2001) Blood 97:1809.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse PD-1 Antibody

There are currently no reviews for this product. Be the first to review Mouse PD-1 Antibody and earn rewards!

Have you used Mouse PD-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review