Mouse PD-1 Fluorescein-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse PD-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human PD‑1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-1
    Accession # Q02242
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PD‑1 in Mouse CD4+ T-Cells by Flow Cytometry. Mouse CD4+ T-cells were stained with Goat Anti-Mouse PD‑1 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1021F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PD-1
Programmed Death-1 (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for the regulation of immune responses (1). Other members of this family include CD28, BTLA, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely plays an inhibitory role in the regulation of immune responses (1-4).
  • References:
    1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
    2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
    3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
    4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
    5. Latchman Y. et al. (2001) Nat. Immunol. 2:261.
    6. Tamura, H. et al. (2001) Blood 97:1809.
  • Long Name:
    Programmed Death-1
  • Entrez Gene IDs:
    5133 (Human); 18566 (Mouse); 102123659 (Cynomolgus Monkey)
  • Alternate Names:
    CD279 antigen; CD279; hPD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Fluorescein-conjugated Control

Ctrl IC108F 1
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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