Mouse PILR-alpha PE-conjugated Antibody

FAB4318P has been discontinued. View all PILR-alpha products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects mouse PILR-alpha in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse PILR-beta is observed and 5% cross-reactivity with recombinant human PILR-alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PILR-alpha isoform 1 (R&D Systems, Catalog # 4318-PR)
    Accession # Q2YFS3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of PILR‑ alpha in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse PILR‑ alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB4318P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PILR-alpha

PILR-alpha (Paired Immunoglobulin-Like type 2 Receptor-alpha) also known as FDF03, is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-beta and it likely gave rise to PILR-beta through gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-alpha and -beta should not be confused with the similarly named PIRs (also paired immunoglobulin‑like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig‑like domains in their extracellular region, PILR-alpha and -beta show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-alpha is a monomeric, 271 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a long, 83 aa cytoplasmic region. The ECD shows one V‑type Ig-like domain between aa 39 - 157, while the cytoplasmic region contains two ITIMs (immunoreceptor Tyr-based inhibitory motifs) between aa 265-270 and 294‑299. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes mouse PILR-alpha an inhibitory receptor. In human, activation of PILR-alpha inhibits CD32/Fc gamma RII-induced calcium mobilization (3). Although CD99 is a known ligand for both PILR-alpha and -beta (4), highest affinity binding seems to occur between CD99 and PILR-alpha (4). Mouse PILR-alpha is found on neutrophils and macrophages (4). Mouse PILR-alpha ECD shares 43% and 69% aa sequence identity with human and rat PILR-alpha ECD, respectively; it shares 75% aa sequence identity with the ECD of mouse PILR-beta (3). One potential isoform of PILR-alpha has been reported. It varies only within the first 28 aa of the signal sequence (5).

  • References:
    1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
    2. Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
    3. Fournier, N. et al. (2000) J. Immunol. 165:1197.
    4. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
    5. SwissProt # Q2YFS3.
  • Long Name:
    Paired-Ig-likeType 2 Receptor alpha
  • Entrez Gene IDs:
    29992 (Human); 231805 (Mouse); 288568 (Rat)
  • Alternate Names:
    Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha
Related Research Areas


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