Mouse PIR-B PerCP-conjugated Antibody

FAB2754C has been discontinued. View all PIR-B products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.
  • Source
    Monoclonal Rat IgG2A Clone # 326414
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
    Gly24-Gly635
    Accession # AAH26937
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Anti-Mouse CD19 PE‑conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse PIR‑B PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2754C) or (B) Rat IgG2A PerCP Isotype Control (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PIR-B
Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.
  • Long Name:
    Paired-Ig-like Receptor B
  • Entrez Gene IDs:
    18733 (Mouse); 65146 (Rat)
  • Alternate Names:
    LILRB3; PIRB; PIR-B
Related Research Areas

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