Mouse R-Spondin 1 Antibody

Catalog # Availability Size / Price Qty
AF3474
AF3474-SP
R‑Spondin 1 in Mouse Ovary.
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Product Details
Citations (5)
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Mouse R-Spondin 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant human R-Spondin 1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) R-Spondin 3 and rmR-Spondin 4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse R-Spondin 1 (R&D Systems, Catalog # 3474-RS)
Ser21-Gly209
Accession # Q9Z132
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry R‑Spondin 1 in Mouse Ovary. View Larger

R‑Spondin 1 in Mouse Ovary. R‑Spondin 1 was detected in immersion fixed frozen sections of E13.5 mouse ovary using Goat Anti-Mouse R‑Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3474) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that belongs to the R-Spondin family (1, 2). R-Spondins share around 40% aa identity. All regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1 - 3). Like other R-spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (amino acids (aa) 34 - 135) followed by a thrombospondin (TSP-1) motif (aa 147 - 207) and a region rich in basic residues (aa 211 - 263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). R-Spondin 1 contains one potential N-glycosylation site. Over aa 21 - 209, mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (6, 7). Disruption is also associated with palmoplantar keratosis (6, 7). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (8). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (8, 9). R-Spondin 1 appears to regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptor, Kremen (10). This competition reduces internalization of DKK-1/LRP-6/Kremen complexes (10). Reports differ on whether R‑Spondin 1 binds LRP-6 directly (10 - 12).

References
  1. Lowther, W. et al. (2005) J. Virol. 79:10093.
  2. Kim, K-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  5. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  6. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
  7. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  8. Kim, K-A. et al. (2005) Science 309:1256.
  9. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  10. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  11. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
  12. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
Long Name
Roof Plate-specific Spondin 1
Entrez Gene IDs
284654 (Human); 192199 (Mouse)
Alternate Names
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

Product Datasheets

Citations for Mouse R-Spondin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016;7(39):63767-63778.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC paraffin embedded
  2. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015;10(4):e0124213.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  3. Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice.
    Authors: Saha S, Bhanja P, Kabarriti R
    PLoS ONE, 2012;6(9):e24072.
    Species: Mouse
    Sample Types: Serum
    Applications: WB
  4. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
    Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
    PLoS ONE, 2011;6(1):e16366.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell mass.
    Authors: Wong VS, Oh AH, Chassot AA, Chaboissier MC, Brubaker PL
    Diabetologia, 2011;54(7):1726-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

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