Mouse R-Spondin 1 Antibody Summary
Accession # Q9Z132
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
R‑Spondin 1 in Mouse Ovary. R‑Spondin 1 was detected in immersion fixed frozen sections of E13.5 mouse ovary using Goat Anti-Mouse R‑Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3474) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 1
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that belongs to the R-Spondin family (1, 2). R-Spondins share around 40% aa identity. All regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1 - 3). Like other R-spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (amino acids (aa) 34 - 135) followed by a thrombospondin (TSP-1) motif (aa 147 - 207) and a region rich in basic residues (aa 211 - 263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). R-Spondin 1 contains one potential N-glycosylation site. Over aa 21 - 209, mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (6, 7). Disruption is also associated with palmoplantar keratosis (6, 7). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (8). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (8, 9). R-Spondin 1 appears to regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptor, Kremen (10). This competition reduces internalization of DKK-1/LRP-6/Kremen complexes (10). Reports differ on whether R‑Spondin 1 binds LRP-6 directly (10 - 12).
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- Parma, P. et al. (2006) Nat. Genet. 38:1304.
- Kim, K-A. et al. (2005) Science 309:1256.
- Zhao, J. et al. (2007) Gastroenterology 132:1331.
- Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
- Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
- Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
Citations for Mouse R-Spondin 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
Sample Types: Whole Tissue
Applications: IHC paraffin embedded
R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
PLoS ONE, 2015;10(4):e0124213.
Sample Types: Cell Lysates
Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice.
Authors: Saha S, Bhanja P, Kabarriti R
PLoS ONE, 2012;6(9):e24072.
Sample Types: Serum
Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
PLoS ONE, 2011;6(1):e16366.
Sample Types: Cell Lysates
R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell mass.
Authors: Wong VS, Oh AH, Chassot AA, Chaboissier MC, Brubaker PL
Sample Types: Tissue Homogenates
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