Mouse Rae-1 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse Rae‑1 alpha / beta / gamma. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha, beta and gamma. It does not recognize Rae-1δ or epsilon.
  • Source
    Monoclonal Rat IgG2A Clone # 199215
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1758A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Rae-1

The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.

  • Long Name:
    Retinoic Acid Early Transcript 1
  • Entrez Gene IDs:
    8480 (Human); 19368 (Mouse)
  • Alternate Names:
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Mouse tumor vasculature expresses NKG2D ligands and can be targeted by chimeric NKG2D-modified T cells.
    Authors: Zhang T, Sentman C
    J Immunol, 2013;190(5):2455-63.
    Species: Mouse
    Sample Type: whole cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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