Mouse Rae-1 epsilon PE-conjugated Antibody

FAB1135P has been discontinued. View all Rae-1 epsilon products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma.
  • Source
    Monoclonal Rat IgG2A Clone # 205001
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rae‑1 epsilon in BaF3 Mouse Cell Line Transfected with Mouse Rae‑1 epsilon by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse Rae‑1 epsilon (top) or Rae-1 gamma (bottom, negative control) was stained with Rat Anti-Mouse Rae‑1 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB1135P). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Rae-1 epsilon

Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88-95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI-linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10-14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1-7).

  • References:
    1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
    2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
    3. Cerwenka, A. et al. (2000) Immunity 12:721.
    4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
    5. Diefenbach, A. et al. (2001) Nature 413:165.
    6. Champsaur, M. et al. (2010) J. Immunol. 185:157.
    7. Markiewicz M. et al. (2012) Immunity 36:132.
  • Long Name:
    Retinoic Acid Early Transcript 1 epsilon
  • Entrez Gene IDs:
    8480 (Human); 379043 (Mouse)
  • Alternate Names:
    Rae1 epsilon; Rae-1 epsilon
Related Research Areas

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