Detects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma.
Monoclonal Rat IgG2A Clone # 205001
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Rae‑1 epsilon in BaF3 Mouse Cell Line Transfected with Mouse Rae‑1 epsilon by Flow Cytometry.
BaF3 mouse pro-B cell line transfected with mouse Rae‑1 epsilon (top) or Rae-1 gamma (bottom, negative control) was stained with Rat Anti-Mouse Rae‑1 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB1135P). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Rae-1 epsilon
Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88-95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI-linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10-14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1-7).
Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
Cerwenka, A. et al. (2000) Immunity 12:721.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
Have you used Mouse Rae-1 epsilon PE-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.