Mouse/Rat FGF basic Quantikine ELISA Kit

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(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (50 uL), EDTA Plasma (50 uL)
  • Sensitivity
    3.68 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant FGF basic.  This assay cross-reacts 100% with Bovine FGF basic, recombinant Human FGF basic is detectable.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant rat FGF basic and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural FGF basic showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF basic.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1.172.7123.85.0320.5


The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 101 91-110
EDTA Plasma (n=4) 97 84-110
Serum (n=4) 95 84-110
Tissue Lysates (n=4) 97 80-106
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FGF basic were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat FGF basic Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF basic
FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
    • Long Name
      Fibroblast Growth Factor basic
    • Entrez Gene IDs
      2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine);
    • Alternate Names
      basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 2 of 2
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    Sample Type
    1. Effect of Silodosin, an Alpha1A-Adrenoceptor Antagonist, on Ventral Prostatic Hyperplasia in the Spontaneously Hypertensive Rat.
      Authors: Shimizu S, Shimizu T, Tsounapi P, Higashi Y, Martin D, Nakamura K, Honda M, Inoue K, Saito M
      PLoS ONE, 2015;10(8):e0133798.
      Species: Rat
      Sample Type: Tissue Homogenates
    2. Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms.
      Authors: Toyoshima A, Yasuhara T, Kameda M, Morimoto J, Takeuchi H, Wang F, Sasaki T, Sasada S, Shinko A, Wakamori T, Okazaki M, Kondo A, Agari T, Borlongan C, Date I
      PLoS ONE, 2015;10(6):e0127302.
      Species: Rat
      Sample Type: Tissue Homogenates
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    Cell Lysis Buffer 2 (1 x 21 mL)

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    Average Rating: 4 (Based on 1 review)

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    Images Ratings Applications Species Reviewed By Date Details
     Mouse/Rat FGF basic Quantikine ELISA Kit MFB00
    Very Good
      Anonymous 08/23/2016
    Image Details
     Mouse/Rat FGF basic Quantikine ELISA Kit MFB00
    : Mouse/Rat FGF basic Quantikine ELISA Kit [MFB00]


    Sample TestedBone marrow cells

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