Mouse/Rat FGF basic Quantikine ELISA Kit

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MFB00
Mouse/Rat FGF basic Standard Curve
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Citations (6)
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Mouse/Rat FGF basic Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
3.68 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma)
Specificity
Natural and recombinant FGF basic.  This assay cross-reacts 100% with Bovine FGF basic, recombinant Human FGF basic is detectable.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant rat FGF basic and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural FGF basic showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF basic.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 49.5 122 394 45 104 382
Standard Deviation 1.17 2.7 12 3.8 5.03 20.5
CV% 2.4 2.2 3.1 8.4 4.8 5.4

Recovery

The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 101 91-110
EDTA Plasma (n=4) 97 84-110
Serum (n=4) 95 84-110
Tissue Lysates (n=4) 97 80-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FGF basic were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat FGF basic ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF basic

FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name:
Fibroblast Growth Factor basic
Entrez Gene IDs:
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names:
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat FGF basic Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats
    Authors: C Chen, H Li, J Jiang, Q Zhang, F Yan
    Oncotarget, 2017;8(42):72676-72699.
    Species: Rat
    Sample Types: Cell Culture Supernates
  2. Fibroblast deletion of ROCK2 attenuates cardiac hypertrophy, fibrosis, and diastolic dysfunction
    Authors: T Shimizu, N Narang, P Chen, B Yu, M Knapp, J Janardanan, J Blair, JK Liao
    JCI Insight, 2017;2(13):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  3. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  4. Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms.
    Authors: Toyoshima A, Yasuhara T, Kameda M, Morimoto J, Takeuchi H, Wang F, Sasaki T, Sasada S, Shinko A, Wakamori T, Okazaki M, Kondo A, Agari T, Borlongan C, Date I
    PLoS ONE, 2015;10(6):e0127302.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Effect of Silodosin, an Alpha1A-Adrenoceptor Antagonist, on Ventral Prostatic Hyperplasia in the Spontaneously Hypertensive Rat.
    Authors: Shimizu S, Shimizu T, Tsounapi P, Higashi Y, Martin D, Nakamura K, Honda M, Inoue K, Saito M
    PLoS ONE, 2015;10(8):e0133798.
    Species: Rat
    Sample Types: Tissue Homogenates
  6. IGF2 ameliorates amyloidosis, increases cholinergic marker expression and raises BMP9 and neurotrophin levels in the hippocampus of the APPswePS1dE9 Alzheimer's disease model mice.
    Authors: Mellott T, Pender S, Burke R, Langley E, Blusztajn J
    PLoS ONE, 2014;9(4):e94287.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Mouse/Rat FGF basic Quantikine ELISA Kit
By Anonymous on 08/23/2016
Sample Tested: Bone marrow cells