|Detection of Mouse and Rat Notch‑2 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and rat embryonic hippocampal neuron tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Mouse/Rat Notch‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5196) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Notch‑2 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.|
Notch proteins (so named for notches in fly wings) are type I transmembrane glycoproteins involved in specifying cell fates and defining boundaries between different cell types during developmental processes. Notch extracellular domains are large, having 34-36 EGF-like repeats followed by three notch/Lin-12 repeats. Notch proteins interact with transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand binding, a series of cleavage events results in the release of the Notch intracellular domain (NICD), which translocates to the nucleus and initiates transcription of Notch-responsive genes. Thus, Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.
The four mammalian Notch receptors appear to have distinct functions, since they do not compensate for one another in genetic studies. Mutations in Notch receptors also lead to specific developmental disorders. For example, Notch-3 is predominantly expressed in the developing central nervous system of mice. Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia.
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