Mouse/Rat Notch-2 Antibody

Discontinued Product

AF5196 has been discontinued.
Detection of Mouse and Rat Notch‑2 by Western Blot.
1 Image
Product Details
FAQs
Reviews

Mouse/Rat Notch-2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Notch‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Notch-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Notch-1 and rmNotch-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Notch‑2
Leu26-Val528
Accession # NP_035058
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse and Rat Notch‑2 by Western Blot. View Larger

Detection of Mouse and Rat Notch‑2 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and rat embryonic hippocampal neuron tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Mouse/Rat Notch‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5196) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Notch‑2 at approximately 275 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-2

Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four Notch homologues involved in development (1‑3). Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, demonstrating that not all functions overlap (4, 5). Mice with hypomorphic Notch-2 show defects in development of kidney, heart and eye (6). Notch-2 is upregulated in mature B cells and is critical for differentiation to splenic marginal zone B cells (7). Notch-2 is also preferentially expressed in choroid plexus epithelia and neuronal precursors (8, 9). Mouse Notch-2 is synthesized as a 2470 amino acid (aa) precursor that contains a 25 aa signal sequence, a 1652 aa extracellular domain (ECD), a 21 aa transmembrane (TM) segment, and a 772 aa cytoplasmic domain. The ECD contains 35 EGF‑like repeats and three Lin-12/Notch repeats, while the cytoplasmic region shows six ankyrin repeats, a glutamine‑rich domain and a PEST sequence. Binding of ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats of human Notch-1 (corresponding to aa 413‑492 in mouse Notch-2) (10). Notch receptors undergo post‑translational furin-type proteolytic cleavage (11). This forms a heterodimer through the interaction of a hydrophobic area in the ligand‑binding extracellular region with the TM/cytoplasmic portion (11, 12). Upon ligand binding, additional sequential proteolysis by
TNF‑converting enzyme (ADAM‑17) and the presenilin‑dependent gamma ‑secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch‑responsive genes (13). Mouse Notch-2 ECD (aa 26‑528) shares 93%, 96%, 92% and 92% aa identity with the corresponding regions of human, rat, canine, and bovine Notch-2, respectively. This region also exhibits 55‑58% aa identity with mouse Notch-1 and Notch-3.

References
  1. Lardelli, M. and U. Lendahl (1993) Exp. Cell Res. 204:364.
  2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
  3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
  4. Hamada, Y. et al. (1999) Development 126:3415.
  5. Shimizu, K. et al. (2002) Biochem. Biophys. Res. Commun. 291:775.
  6. McCright, B. et al. (2001) Development 128:491.
  7. Saito, T. et al. (2003) Immunity 18:675.
  8. Irvin, D. K. et al. (2001) J. Comp. Neurol. 436:167.
  9. Solecki, D. J. et al. (2001) Neuron 31:557.
  10. Hambleton, S. et al. (2004) Structure 12:2173.
  11. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
  12. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
  13. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Entrez Gene IDs
4853 (Human); 29492 (Rat)
Alternate Names
AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse/Rat Notch-2 Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat Notch-2 Antibody and earn rewards!

Have you used Mouse/Rat Notch-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review