Detection of SIRP alpha /CD172a in PC‑12 Rat Cell Line by Flow Cytometry.
PC‑12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRP alpha /CD172a PE‑conjugated Monoclonal Antibody (Catalog # FAB7307P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SIRP alpha /CD172a in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse Integrin alpha M/CD11b APC‑conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Mouse Anti-Mouse/Rat SIRP alpha /CD172a PE‑conjugated Monoclonal Antibody (Catalog # FAB7307P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.
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