Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody FAB7089N: R&D Systems

Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 713412
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
    Accession # O08530
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and GFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with either (A) Rat Anti-Mouse S1P1/EDG‑1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB7089N) or (B) Rat IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7089N) or (B) Rat IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P1/EDG-1
S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.
  • Long Name:
    Endothelial Differentiation Gene 1
  • Entrez Gene IDs:
    1901 (Human); 13609 (Mouse)
  • Alternate Names:
    CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Flow Mouse Anonymous 08/24/2017
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Summary

ApplicationFlow Cytometry
Sample TestedAdult splenocytes,Bone marrow,Thymocytes
SpeciesMouse

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