Mouse S1P1/EDG-1 Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 713412
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
    Accession # O08530
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
Immunohistochemistry
S1P1/EDG‑1 in Mouse Heart. S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S1P1/EDG-1
S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.
  • Long Name:
    Endothelial Differentiation Gene 1
  • Entrez Gene IDs:
    1901 (Human); 13609 (Mouse)
  • Alternate Names:
    CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Macrophages Induce Long-Term Trapping of ?? T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State
    Authors: A Audemard-V, M Rivière, A Durand, E Peranzoni, V Guichard, P Hamon, N Bonilla, T Guilbert, A Boissonnas, C Auffray, G Eberl, B Lucas, B Martin
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment
    Authors: MJ Lang, M Mori, J Ruer-Laven, J Pieters
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells
    Authors: A Mendoza, V Fang, C Chen, M Serasinghe, A Verma, J Muller, VS Chaluvadi, ML Dustin, T Hla, O Elemento, JE Chipuk, SR Schwab
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. CD8?? intraepithelial lymphocytes arise from two main thymic precursors
    Authors: R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.
    Authors: Xu, Shengli, Ou, Xijun, Huo, Jianxin, Lim, Kristen, Huang, Yuhan, Chee, Sheena, Lam, Kong-Pen
    Nat Commun, 2015;6(0):6764.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. IL-17-Producing Vgamma4+ gammadelta T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions.
    Authors: Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K
    J Immunol, 2015;195(4):1408-16.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors.
    Authors: Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K
    J Exp Med, 2014;211(13):2583-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
    Authors: Arnon, Tal I, Horton, Robert M, Grigorova, Irina L, Cyster, Jason G
    Nature, 2013;493(7434):684-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Moesin controls clathrin-mediated S1PR1 internalization in T cells.
    Authors: Nomachi, Akira, Yoshinaga, Masanori, Liu, Jaron, Kanchanawong, Pakorn, Tohyama, Kiyoshi, Thumkeo, Dean, Watanabe, Takeshi, Narumiya, Shuh, Hirata, Takako
    PLoS ONE, 2013;8(12):e82590.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. The sphingosine 1-phosphate receptor S1P(2) maintains the homeostasis of germinal center B cells and promotes niche confinement.
    Authors: Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG
    Nat. Immunol., 2011;12(7):672-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Cell and Tissue Staining Kits
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Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
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Immunohistochemistry Reagents
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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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NorthernLights Guard Mounting Media

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VisUCyte Antigen Retrieval Reagent-Acidic

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VisUCyte Antigen Retrieval Reagent-Basic

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VisUCyte Antigen Retrieval Reagent-Universal

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Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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