Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with Rat Anti-Mouse S1P1/EDG‑1 Biotinylated Monoclonal Antibody (Catalog # FAB7089B) followed by Streptavidin-Allophycocyanin (Catalog # F0050). Quadrant markers were set based on control antibody staining (Catalog # IC006B).
Detection of S1P1/EDG‑1 in Mouse thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse S1P1/EDG‑1 Biotinylated Monoclonal Antibody (Catalog # FAB7089B) followed by Streptavidin-Allophycocyanin (Catalog # F0050) and Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # IC006B).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40
kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1
is one of five receptors for the bioactive lipid S1P and mediates most of S1P
effects on angiogenesis, vascular maturation, and cell migration, especially T
cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid
identity within the N-terminal extracellular portion used as an immunogen.
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