Detection of Mouse S1P5/EDG‑8 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mouse S1P5. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse S1P5/EDG‑8 Monoclonal Antibody (Catalog # MAB9084) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for S1P5/EDG‑8 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP were stained with and either (A) Rabbit Anti-Mouse S1P5/EDG‑8 Monoclonal Antibody (Catalog # MAB9084) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
S1P5 (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P1 -S1P4 (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P5 is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P5 is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C+ (in human CD14+CD16-) monocytes, pre- and mature oligodendrocytes, and CD27-CD116+ (mature) NK cells. S1P5 appears to play a role in cell trafficking. On monocytes and NK cells, S1P5 promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P5 also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P5 appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P5 shares 96% and 94% aa sequence identity with rat and human S1P5 extracellular domains, respectively.
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