Mouse Semaphorin 4C APC-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects HEK293 human embryonic cell line transfected with mouse Semaphorin 4C by flow cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Rat IgG1 Clone # 663203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myelona cell line NS0-derived recombinant mouse Semaphorin 4C
    Glu22-Gly664
    Accession # Q64151
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4C APC‑conjugated Monoclonal Antibody (Catalog # FAB8497A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4C
Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.
  • Entrez Gene IDs:
    54910 (Human); 20353 (Mouse); 301346 (Rat)
  • Alternate Names:
    FLJ20369; KIAA1739; MGC126382; MGC126383; M-Sema F; M-SEMA-F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; Semaf; SEMAI; Semaphorin 4C; semaphorin-4C
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 APC-conjugated Isotype Control

Ctrl IC005A
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Mouse Alaa Amash 07/27/2016
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Summary

ApplicationFlow Cytometry
Sample TestedSplenic B cells
SpeciesMouse

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