|Detection of Semaphorin 4D/CD100 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Semaphorin 4D/CD100 PE‑conjugated Monoclonal Antibody (Catalog # FAB52351P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
Semaphorin 4D (Sema4D) also known as CD100, is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.
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