Mouse Siglec-G APC-conjugated Antibody

FAB7775A has been discontinued. View all Siglec-G products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Siglec-G in ELISA. In direct ELISA, no cross-reactivity with recombinant mouse Siglec-F or recombinant human Siglec-10 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 805903
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Siglec-G
    Glu20-Gln525
    Accession # NP_766488
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Siglec-G in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin  alpha M/CD11b PE‑conjugated Monoclonal Antibody (Catalog # FAB1124P) and either (A) Rat Anti-Mouse Siglec-G APC‑conjugated Monoclonal Antibody (Catalog # FAB7775A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-G
Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type Ig-like domain which mediates sialic acid binding, followed by varying numbers of C2-type Ig-like domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120 kDa, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269 aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares approximately 63% aa sequence identity with human Siglec-10 within the extracellular domain.
  • Long Name:
    Sialic Acid Binding Ig-like Lectin G
  • Entrez Gene IDs:
    243958 (Mouse)
  • Alternate Names:
    Siglec-10; SiglecG; Siglec-G
Related Research Areas

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