Mouse SLAM/CD150 Fluorescein-conjugated Antibody

FAB4330F has been discontinued. View all SLAM/CD150 products.
  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
FAQs
Reviews
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse SLAM/CD150.
  • Source
    Monoclonal Rat IgG2A Clone # 459911
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
    Thr25-Pro242
    Accession # Q9QUM4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of SLAM/CD150 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin for 2 days were stained with Rat Anti-Mouse SLAM/CD150 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4330F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

  • References:
    1. Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
    2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
    3. Cocks, B.G. et al. (1995) Nature 376:260. 
    4. Wang, N. et al. (2004) J. Exp. Med. 199:1255. 
    5. Hahm, B. et al. (2004) Virology 323:292. 
    6. Nanda, N. et al. (2005) Blood 106:3028. 
    7. Kiel, M.J. et al. (2005) Cell 121:1109. 
    8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
    9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
    10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
    11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
    12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
    13. Tatsuo, H. et al. (2000) Nature 406:893.
    14. Hsu, E.C. et al. (2001) Virology 279:9.
  • Long Name:
    Signaling Lymphocytic Activation Molecule
  • Entrez Gene IDs:
    6504 (Human); 27218 (Mouse)
  • Alternate Names:
    CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Deficiency in Aryl Hydrocarbon Receptor (AHR) Expression throughout Aging Alters Gene Expression Profiles in Murine Long-Term Hematopoietic Stem Cells.
    Authors: Bennett J, Singh K, Unnisa Z, Welle S, Gasiewicz T
    PLoS ONE, 2015;10(7):e0133791.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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