Mouse TGF-beta 1 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TGF-beta 1.
  • Source
    Monoclonal Rat IgG2A Clone # 860206
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse TGF-beta 1
    Met1-Ser390
    Accession # P04202
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TGF‑ beta 1 in Mouse iTreg cells by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 μg/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 μg/mL Recombinant Mouse IL‑2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1
TGF-beta 1 (Transforming Growth Factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa Latency‑Associated Peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI, also known as Activin Receptor‑Like Kinase 5(ALK‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors Betaglycan (also known as TGF‑ beta  RIII) and Endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
  • References:
    1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
    2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
    3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
    4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
    5. Lin, J.S. et al. (2006) Reproduction 132:179.
    6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
    7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
    8. Derynck, R. et al. (1985) Nature 316:701.
    9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
    10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
    11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
    12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
  • Long Name:
    Transforming Growth Factor beta 1
  • Entrez Gene IDs:
    7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
  • Alternate Names:
    CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Culture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4(+)CD25(+)Foxp3 T cells
    Authors: HS Yu, MK Park, SA Kang, KS Cho, SJ Mun, HJ Roh
    Stem Cell Res Ther, 2017;8(1):8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  2. Suppressive IL-17A(+)Foxp3(+) and ex-Th17 IL-17A(neg)Foxp3(+) Treg cells are a source of tumour-associated Treg cells
    Authors: S Downs-Cann, S Berkey, GM Delgoffe, RP Edwards, T Curiel, K Odunsi, DL Bartlett, N Obermajer
    Nat Commun, 2017;8(0):14649.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
    Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
    Cancer Res., 2016;76(10):3045-56.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
    Authors: Lee Y, Park H, Woo S, Park E, Kang J
    J Pharmacol Exp Ther, 2014;350(3):563-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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