Mouse TREML2/TLT-2 APC-conjugated Antibody

FAB6707A has been discontinued and is replaced by FAB6707R.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TREML2/TLT-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) TREM-1, -2, -2b, -3, -4, rmTREML1/TLT-1, rmTREML4/TLT-4, or recombinant human (rh) NKp44 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 656906
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse TREML2/TLT-2
    His25-Ala270
    Accession # Q2LA85
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of TREML2/TLT‑2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Rat Anti-Mouse TREML2/TLT‑2 APC‑conjugated Monoclonal Antibody (Catalog # FAB6707A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREML2/TLT-2
TREML2 (Triggering Receptor Expressed on Myeloid cells-like 2), alsol known as TLT2, is a 42-45 kDa member of the TREM family of immune modulatory proteins. In mouse, it is a 306 amino acid (aa) type I transmembrane glycoprotein that contains a 246 aa extracellular domain (ECD). TREML2 is expressed by CD5+ B-1 type B cells, marginal zone IgMhi IgDlo B-2 type B cells, macrophages, microglia and neutrophils; it is absent from T cells and monocytes (although human monocytes strongly express TREML2). While it is unclear what, or if, a protein ligand for TREML2 exists, it is known to bind phosphatidylserine and initiate efferocytosis (phagocytosis of apoptotic cells). TREML2 expression is promoted by TLR3 ligation, and once expressed and "activated", it appears to potentiate downstream effects that arise from independent GPCR activation, such as inflammatory mediator release and chemotaxis following chemokine binding to CXC receptors. Over aa 25-270 (the ECD), mouse TREML2 shares 78% and 52% aa sequence identity with rat and human TREML2, respectively. With respect to mouse TREML2 ECD similarity to mouse TREM2 and TREML1 ECD, there is less than 20% aa sequence identity for each.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells-like 2
  • Entrez Gene IDs:
    79865 (Human); 328833 (Mouse)
  • Alternate Names:
    C6orf76; dJ238O23.1; FLJ13693; MGC149715; MGC149716; TLT2; TLT-2; TREML2; trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like 2; triggering receptor expressed on myeloid cells-like protein 2; UNQ6268/PRO20473
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Mouse TREML2/TLT-2 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee