Mouse TREML4/TLT-4 PE-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TREML4/TLT-4 in ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 817914
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT-4
    Gly25-Pro195
    Accession # Q3LRV9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of TREML4/TLT‑4 in Mouse CD8+ CD11+c Splenocytes by Flow Cytometry. Mouse CD8+ CD11+c splenocytes were stained with Rat Anti-Mouse TREML4/TLT‑4 PE‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB5500P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREML4/TLT-4

TREML4 (Triggering receptor expressed on myeloid cells-like 4; also TLT4) is a 53-55 kDa member of the Ig superfamily.  It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor.  Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length.  It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue.  There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence.  Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells-like 4
  • Entrez Gene IDs:
    285852 (Human); 224840 (Mouse); 680862 (Rat)
  • Alternate Names:
    TLT4; TREML4

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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