Detection of TREML4/TLT‑4 in Mouse CD8+ CD11+c Splenocytes by Flow Cytometry.
Mouse CD8+ CD11+c splenocytes were stained with Rat Anti-Mouse TREML4/TLT‑4 PE‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB5500P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TREML4 (Triggering receptor expressed on myeloid cells-like
4; also TLT4) is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and
based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane
(TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa
extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the
TM segment contains one Lys residue. There is one splice variant that shows a
deletion of aa 21-24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent,
and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat
TREML4, but only 52% aa sequence identity with human TREML4.
Triggering Receptor Expressed on Myeloid Cells-like 4
Entrez Gene IDs:
285852 (Human); 224840 (Mouse); 680862 (Rat)
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