Detects mouse VEGF R1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.
Monoclonal Rat IgG2B Clone # 141522
Protein A or G purified from hybridoma culture supernatant
Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4711A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF R1/Flt-1
VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.
Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.
He, Y. et al. (1999) Molecular Endocrinology 13:537.
Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.
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Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis Authors: LA Murray, DM Habiel, M Hohmann, A Camelo, H Shang, Y Zhou, AL Coelho, X Peng, M Gulati, B Crestani, MA Sleeman, T Mustelin, MW Moore, C Ryu, AD Osafo-Addo, JA Elias, CG Lee, B Hu, JD Herazo-May, DA Knight, CM Hogaboam, EL Herzog JCI Insight, 2017;2(16):. Species: Mouse Sample Type: Whole Cells Application: Flow
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