Mouse VEGF R2/KDR/Flk-1 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse VEGF R2/KDR/Flk‑1 in flow cytometry.
  • Source
    Monoclonal Rat IgG1 Clone # 522302
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2/KDR/Flk‑1
    Accession # P35918
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of VEGF R2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R2/KDR/Flk‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4432A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF R2/KDR/Flk-1

VEGF R2, also known as KDR, Flk-1, and CD309, is a 210-220 kDa type I transmembrane glycoprotein member of the 7-Ig/Class V subfamily that belongs to the tyrosine protein kinase family of molecules (1, 2). It is principally expressed on endothelial cells (EC), but also appears on neurons, mast cells, immature dendritic cells and monocytes (3-6). VEGF R2 serves as the major proangiogenic switch that regulates blood vessel development and repair (1, 2). It is known to bind VEGF-A, -C, and -D. On the cell surface, VEGF R2 either homodimerizes or heterodimerizes with VEGF R3 (1). As a homodimer, it appears to interact with Neuropilin-1 (7). It also interacts with Integrin beta 3 subunit, either through its extracellular domain or cytoplasmic tail (8). This interaction may be necessary for EC activation. Notably, VEGF R2 can also be activated in the absence of a VEGF ligand when under torsion stress and in a complex with CD31 and VE-Cadherin (9). Over amino acids 20-762, mouse VEGF R2 shares 93% and 80% amino acid sequence identity with rat and human VEGF R2, respectively.

  • References:
    1. Smith, G.A. et al. (2015) Biosci. Rep. Aug 18 [ePub ahead of print].
    2. Shibuya, M. (2013) J. Biochem. 153:13.
    3. Le, X-F. et al. (2008) Cell Cycle 7:3747.
    4. Okabe, K. et al. (2014) Cell 159:584.
    5. Detoraki, A. et al. (2009) J. Allergy Clin. Immunol. 123:1142.
    6. Dong, Z. et al. (2009) Curr. Pharm. Des. 15:365.
    7. Plein, A. et al. (2014) Microcirculation 21:315.
    8. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
    9. DuToit, A. (2015) Nat. Rev. Mol. Cell Biol. 16:268.
  • Long Name:
    Vascular Endothelial Growth Factor Receptor 2
  • Entrez Gene IDs:
    3791 (Human); 16542 (Mouse)
  • Alternate Names:
    CD309 antigen; CD309; EC 2.7.10; EC; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 APC-conjugated Isotype Control

Ctrl IC005A
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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