|Detection of VEGF R3/Flt‑4 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse VEGF R3/Flt‑4 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB743G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.|
VEGF R3 (Flt-4), together with VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
In adults, VEGF R3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
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