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Nicotinamide

Catalog #: 4106
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4106/50

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Nicotinamide | CAS No. 98-92-0 | Poly(ADP-ribose) Polymerase Inhibitors
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Description: PARP-1 inhibitor

Chemical Name: Pyridine-3-carboxamide

Purity: ≥99%

Product Details
Citations (6)
Reviews
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

Nicotinamide is an anti-inflammatory agent. Inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. NAD+ precursor. Promotes differentiation of mesenchymal stem cells to insulin producing cells when used in combination with growth factors and high glucose concentration. Nicotinamide also acts as a SIRT1 inhibitor and promotes the expansion of hematopoietic progenitor cells. Nicotinamide can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells.

Nicotinamide synthesized to Ancillary Material Grade also available.

For more information about how Nicotinamide may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Scientific Data

N/A iPSC-derived Human Intestinal Organoid View Larger

Application of Nicotinamide in Culture and Characterization of iPSC-derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were cultured using Nicotinamide, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).

N/A Adult Stem Cell-derived Lung Organoids View Larger

Application of Nicotinamide in Culture and Characterization of Adult Stem Cell-derived Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes Nicotinamide, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), SB 202190 (Catalog # 1264), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) a Human Cytokeratin 5 (KRT5) Antibody (green) and a Human p63/TP73L Antibody (Catalog # AF1916; red) to visualize basal cells, (B) a Mouse Podoplanin (PDPN) Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Human p63/TP73L Antibody (Catalog # AF1916; red) to visualize basal cells, and (C, D) a Mouse MUC5AC Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Human/Mouse/Rat SOX2 Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

N/A Adult Stem Cell-derived Liver Organoids View Larger

Application of Nicotinamide in Culture and Characterization of Adult Stem Cell-derived Liver Organoids. Adult stem cell-derived liver organoids were cultured using Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-0 (Catalog # 2939), Forskolin (Catalog # 1099), N-Acetylcysteine (Catalog # 7874), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Nicotinamide, HEPES (Catalog # 3173), N-Acetylcysteine (Catalog # 7874), A 83-0 (Catalog # 2939). Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3 beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

N/A Adult Stem Cell-derived Human Descending Colon Organoids View Larger

Application of Nicotinamide in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids. Adult stem cells isolated from human descending colon were embedded and then cultured for 30 days in intestinal organoid culture medium, which includes Nicotinamide, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), N-Acetylcysteine (Catalog # 7874), A 83-01 (Catalog # 2939), along with other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Human MUC2 Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Human/Mouse E-Cadherin Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). (B) Organoids were fixed and stained with a Human Chromogranin A Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Human/Mouse E-Cadherin Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue).

Technical Data

M.Wt:
122.12
Formula:
C6H6N2O
Solubility:
Soluble to 100 mM in water
Purity:
≥99%
Storage:
Store at RT
CAS No:
98-92-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Citations for Nicotinamide

The citations listed below are publications that use Tocris products. Selected citations for Nicotinamide include:

6 Citations: Showing 1 - 6

  1. INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury.
    Authors: Steven L Et al.
    Nat Commun  2022;13:6037
  2. Cell-type-specific regulation of neuronal intrinsic excitability by macroautophagy.
    Authors: Emanuela Et al.
    Elife  2020;9
  3. Evaluation of Compound Activity in Primary Human Intestinal Organoids Using Gene Expression and Histology.
    Authors: Mihee M Et al.
    Curr Protoc Pharmacol  2019;85:e54
  4. Inhibition of APE1-endonuclease activity affects cell metabolism in colon cancer cells via a p53-dependent pathway.
    Authors: Mark R Et al.
    DNA Repair (Amst)  2019;82:102675
  5. Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity.
    Authors: Thomas C Et al.
    Cell Stem Cell  2019;25:103-119.e6
  6. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.
    Authors: Jun Et al.
    Cell  2017;169:243-257.e25

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