p27/Kip1 IHC Detection Kit

The cyclin-dependent kinase (cdk) inhibitor p27, also known as Kip1, is a tumor suppressor protein. Loss of p27 function has been linked to genetic instability and the progression of many types of cancer. R&D Systems’ Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256) includes all reagents required for excellent immunoreactivity and localization of the human p27 antigen in formalin-fixed tissues.

The antibody included detects p27 in human and mouse tissues. Specificity was confirmed by comparing staining on tissue sections of normal versus p27 knockout mice and by Western blot on human prostate tissue lysates. Heat-Induced Epitope Retrieval (HIER) pretreatment is required for the detection of this antigen in formalin-fixed, paraffin-embedded (FFPE) sections of human and mouse normal and cancer tissues.

Features

• Optimized staining reagents
• Highly consistent
• Suitable for high-throughput clinical studies

Kit Contents

• Antigen Retrieval Reagents
• Blocking Reagents
• Biotinylated Primary Antibody
• HRP/DAB Visualization Reagents

p27 in Human Prostate Cancer. p27 was detected in paraffin-embedded human prostate cancer sections using R&D Systems’ Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256). Tissue was stained using anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue). See IHC Enzymatic Protocol 1.0.