Pepstatin A Janelia Fluor® 526

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Pepstatin A Janelia Fluor® 526 | CAS No. 2410614-07-0 | Fluorescent Lysosome Probes
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Description: Fluorogenic green-emitting lysosome tracker and stain
Alternative Names: Pepstatin A JF526

Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate

Purity: ≥90%

Product Details

Biological Activity

Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Optical Data for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Green
λabs 530 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Application confocal microscopy, STED, 3D-SIM, lattice light-sheet microscopy
Alternative For LysotrackerTM- Lysotracker is a registered trademark of Thermo Fisher

Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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