Pepstatin A Janelia Fluor® 526

Tocris Bioscience | Catalog # 7314

Fluorogenic green-emitting lysosome tracker and stain. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (STED, 3D-SIM), lattice light-sheet microscopy.
Tocris Bioscience

Key Product Details

Description

Fluorogenic green-emitting lysosome tracker and stain. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (STED, 3D-SIM), lattice light-sheet microscopy.

Alternative Names

Pepstatin A JF526

Wavelength

Excitation = 530 nm, Emission = 549 nm
View all Fluorescent Lysosome Probes »

Product Description

Key information: Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain for live cell imaging.

Used for: fixed and live-cell imaging of lysosomes.

Application: Suitable for confocal microscopy and super resolution microscopy (SRM) including STED and 3D-SIM (structured illumination microscopy), as well as two-color lattice light-sheet microscopy.

Properties and Photophysical Data: No wash step is required when used for multicolor microscopy experiments. Excitation and emission maxima (λ) are 530 nm and 549 nm, respectively; quantum yield = 0.87; extinction coefficient = 118,000 M-1cm-1.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data Images for Pepstatin A Janelia Fluor® 526

Application of Pepstatin A Janelia Fluor® 526 in live U2OS cells.

Application of Pepstatin A Janelia Fluor® 526 in live U2OS cells.

Live U2OS cells were stained with Pepstatin A Janelia Fluor® 526 (2 µM) for 1 hour with Verapamil (10 µM) and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
Application of Pepstatin A Janelia Fluor® 526 in fixed U2OS cells.

Application of Pepstatin A Janelia Fluor® 526 in fixed U2OS cells.

Fixed U2OS cells were stained with Pepstatin A Janelia Fluor® 526 (2 µM) for 1 hour in 0.1 M sodium acetate buffer pH 4.5. Cells were imaged on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Pepstatin A Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Yellow
λabs 530 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Application Confocal Microscopy, STED, 3D-SIM, Lattice Light-sheet Microscopy
Alternative For Lysotracker™- Lysotracker is a registered trademark of Thermo Fisher

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Pepstatin A Janelia Fluor® 526

Molecular Weight

1442.53

Formula

C67H91F6N9O12.CF3CO2H

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate

CAS Number

2410614-07-0

InChI Key

JONSFJPZCPEZFP-WKVYEHBGSA-N

SMILES

OC(C(F)(F)F)=O.CC(C[C@@H]([C@H](CC(NCCCCCCNC(C1=CC(C2=C3C=C(/C(C=C3OC4=C2C=C(C(N5CC(F)(C5)F)=C4)F)=[N+]6CC(F)(C\6)F)F)=C(C([O-])=O)C=C1)=O)=O)O)NC([C@@H](NC(C[C@@H]([C@@H](NC([C@H](C(C)C)NC([C@H](C(C)C)NC(CC(C)C)=O)=O)=O)CC(C)C)O)=O)C)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 14.43 10

Preparing Stock Solutions for Pepstatin A Janelia Fluor® 526

The following data is based on the product molecular weight 1442.53.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 6.93 mL 34.66 mL 69.32 mL
0.5 mM 1.39 mL 6.93 mL 13.86 mL
1 mM 0.69 mL 3.47 mL 6.93 mL
5 mM 0.14 mL 0.69 mL 1.39 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Pepstatin A Janelia Fluor® 526

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pepstatin A Janelia Fluor® 526

For research use only

Customer Reviews for Pepstatin A Janelia Fluor® 526 (1)

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  • Specific dye
    Name: Karthik Krishnamurthy
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: astrocytes
    Verified Customer | Posted 08/03/2021
    labeling of lysosomes in astrocytes under basal conditions
    Pepstatin A Janelia Fluor® 526 7314

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