Phospho-GSK-3 beta (S9) Fluorescein-conjugated Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human GSK-3 beta when phosphorylated at S9.
  • Source
    Monoclonal Mouse IgG1 Clone # 609739
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phosphopeptide containing the human GSK-3 beta S9 site
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of GSK‑3 beta in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line untreated (open histogram) or treated with PMA (filled histogram) was stained with Mouse Anti-Human Phospho-GSK‑3 beta (S9) Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1590F) or isotype control antibody (Catalog # IC002F, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of Glycogen Synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

  • Long Name:
    Glycogen Synthase Kinase 3 beta
  • Entrez Gene IDs:
    2932 (Human); 56637 (Mouse); 84027 (Rat)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. NKG2D receptor regulates human effector T-cell cytokine production.
    Authors: Barber A, Sentman CL
    Blood, 2011;117(24):6571-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. The role of glycogen synthase kinase 3 in regulating IFN-beta-mediated IL-10 production.
    Authors: Wang H, Brown J, Garcia CA, Tang Y, Benakanakere MR, Greenway T, Alard P, Kinane DF, Martin M
    J. Immunol., 2011;186(2):675-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Convergence of the Mammalian Target of Rapamycin Complex 1- and Glycogen Synthase Kinase 3-{beta}-Signaling Pathways Regulates the Innate Inflammatory Response.
    Authors: Wang H, Brown J, Gu Z, Garcia CA, Liang R, Alard P, Beurel E, Jope RS, Greenway T, Martin M
    J. Immunol., 2011;186(9):5217-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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