Phospho-HSP27 (S78/S82) DuoSet IC ELISA

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Assay Length
      4 hours 40 minutes (after plate preparation)
    • Sample Volume Required
      Cell lysates (100 µL)
    • Sufficient Materials
      Kits available for two, five, or fifteen 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat HSP27 (S78 / S82) in cell lysates. An immobilized capture antibody specific for HSP27 binds both phosphorylated and unphosphorylated HSP27. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Available in 2, 5, and 15- (96-well) plate pack sizes
    • Economical alternative to Western blot
    Kit Content
    • Capture Antibody
    • Conjugated Detection Antibody
    • Calibrated Immunoassay Standard or Control
    • Streptavidin-HRP
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Lysis Buffer*

    IC Diluent*

    Blocking Buffer*


    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: HSP27
    The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.
    • Long Name:
      Heat Shock Protein 27
    • Entrez Gene IDs:
      3315 (Human); 15510 (Mouse); 24471 (Rat)
    • Alternate Names:
      28 kDa heat shock protein; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HMN2B; HS.76067; HSP25; HSP27; HSP27HSP 27; HSP28CMT2F; HSPB1; SRP27; Stress-responsive protein 27
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Sample Type
  1. Stromal expression of heat-shock protein 27 is associated with worse clinical outcome in patients with colorectal cancer lung metastases.
    Authors: Schweiger T, Nikolowsky C, Starlinger P, Traxler D, Zimmermann M, Birner P, Hegedus B, Dome B, Bergmann M, Mildner M, Klepetko W, Hoetzenecker K, Ankersmit H
    PLoS ONE, 2015;10(3):e0120724.
    Species: Human
    Sample Type: Serum
  2. Proteomic alterations in heat shock protein 27 and identification of phosphoproteins in ascending aortic aneurysm associated with bicuspid and tricuspid aortic valve.
    Authors: Matt P, Fu Z, Carrel T, Huso D, Dirnhofer S, Lefkovits I, Zerkowski H, Van Eyk J
    J. Mol. Cell. Cardiol., 2007;43(6):792-801.
    Species: Human
    Sample Type: Tissue Homogenates

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack

ELISA DY999 65  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 2

ELISA DY995 18  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Stop Solution 2N Sulfuric Acid

ELISA DY994 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate Sealers

ELISA DY992  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-HRP

ELISA DY998 50  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Clear Polystyrene Microplates

ELISA DY990 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Additive 1

ELISA DY005 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Glo Substrate Reagent Pack

ELISA DY993 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate-coating Buffer

ELISA DY006 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sample Diluent Concentrate 2 (2X)

ELISA DYC002 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sample Diluent Concentrate 1 (5X)

ELISA DYC001 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 3 (5X)

ELISA DY004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Black Polystyrene Microplates, 25 Pack

ELISA DY991 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Phospho-HSP27 (S78/S82) DuoSet IC ELISA?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee