Phospho-Tyrosine PE-conjugated Antibody

  
  • Specificity
    Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross‑reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 179003
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH-coupled phospho-tyrosine synthetic peptide
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Phospho-Tyrosine in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) either (A) untreated or (B) treated with PMA were stained with Mouse Anti-Phospho-Tyrosine PE‑conjugated Monoclonal Antibody (Catalog # IC1676P). Quadrant markers were set based upon isotype control staining (Catalog # IC002P; data not shown). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Phospho-Tyrosine
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal cell cycle progression and oncogenic transformation. The role  of tyrosine phosphorylation is a matter of active and ongoing research. Specific antibodies that  recognize phosphorylated tyrosine residues are valuable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they are involved.
  • Alternate Names:
    2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; Phospho-Tyrosine; pTyrosine; Tyrosine
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 30  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 1
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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