PIWIL2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-41143
![Western Blot: PIWIL2 AntibodyBSA Free [NBP2-41143]](https://beta-resources.rndsystems.com/images/products/PIWIL2-Antibody-Western-Blot-NBP2-41143-img0001.jpg "Western Blot: PIWIL2 AntibodyBSA Free [NBP2-41143]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410 - 460 of PIWI-L2. Amino Acid Squence: TRYNNRTYRIDDVDWNK
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%)
Specificity
At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PIWIL2 Antibody - BSA Free (NBP2-41143) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PIWIL2 Antibody - BSA Free
Western Blot: PIWIL2 AntibodyBSA Free [NBP2-41143]
Western Blot: PIWIL2 Antibody [NBP2-41143] - Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: PIWIL2 Antibody - BSA Free [NBP2-41143] -
Immunocytochemistry/ Immunofluorescence: PIWIL2 Antibody - BSA Free [NBP2-41143] - Immunofluorescence of PIWIL2 in HepG2 cells with PIWIL2 antibody at 5 u/ml.
Immunocytochemistry/ Immunofluorescence: PIWIL2 Antibody - BSA Free [NBP2-41143] -
Immunocytochemistry/ Immunofluorescence: PIWIL2 Antibody - BSA Free [NBP2-41143] - Immunocytochemistry of PIWIL2 in HepG2 cells with PIWIL2 antibody at 2.5 ug/ml.Applications for PIWIL2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIWIL2
Long Name
Piwi-like Protein 2
Alternate Names
CT80, HILI, MILI
Gene Symbol
PIWIL2
Additional PIWIL2 Products
Product Documents for PIWIL2 Antibody - BSA Free
Product Specific Notices for PIWIL2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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