Porcine CXCL8/IL-8 Quantikine ELISA Kit

(15 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL)
  • Sensitivity
    6.7 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant porcine IL-8
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Porcine IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-8 in cell culture supernates and serum. It utilizes E. coli-expressed recombinant porcine IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure or quantify the recombinant porcine IL-8. Results obtained using natural porcine IL-8 show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-8.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Standard Deviation9.464106949108


The recovery of porcine IL-8 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 108 80-120
Serum (n=7) 91 80-100
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of porcine IL-8 in each matrix were diluted with Calibrator Diluent and then assayed.
Porcine CXCL8/IL-8 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL8/IL-8
CXCL8/Interleukin-8 is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. CXCL8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. CXCL8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents CXCL8 interaction with CXCR1.
    • Entrez Gene IDs
      3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline);
    • Alternate Names
      3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 120 µL Substrate Solution
    12.   Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 120 µL Stop Solution
    14.   Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 15
    Filter your results:

    Sample Type
    1. Epithelial proinflammatory response and curcumin-mediated protection from staphylococcal toxic shock syndrome toxin-1.
      Authors: Schaefers MM, Breshears LM, Anderson MJ, Lin YC, Grill AE, Panyam J, Southern PJ, Schlievert PM, Peterson ML
      PLoS ONE, 2012;7(3):e32813.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    2. Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
      Authors: Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A
      Crit. Care Med., 2012;40(1):162-8.
      Species: Porcine
      Sample Type: Serum
    3. Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota.
      Authors: Riboulet-Bisson E, Sturme MH, Jeffery IB
      PLoS ONE, 2012;7(2):e31113.
      Species: Porcine
      Sample Type: Serum
    4. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
      Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
      J. Immunol., 2011;187(9):4913-9.
      Species: Porcine
      Sample Type: Plasma
    5. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
      Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
      Crit. Care Med., 2010;38(6):1467-74.
      Species: Porcine
      Sample Type: Plasma
    6. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
      Authors: McILwain RB, Timpa JG, Kurundkar AR
      Lab. Invest., 2010;90(1):128-39.
      Species: Porcine
      Sample Type: Plasma
    7. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
      Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
      FASEB J., 2010;24(3):712-22.
      Species: Porcine
      Sample Type: Plasma
    8. Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
      Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
      Am. J. Clin. Nutr., 2009;89(5):1393-401.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    9. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
      Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
      J. Immunol., 2008;180(9):6346-53.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    10. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
      Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
      Eur. Respir. J., 2008;32(4):1037-46.
      Species: Porcine
      Sample Type: Serum
    11. Improved pulmonary function by acid sphingomyelinase inhibition in a newborn piglet lavage model.
      Authors: von Bismarck P, Wistadt CF, Klemm K, Winoto-Morbach S, Uhlig U, Schutze S, Adam D, Lachmann B, Uhlig S, Krause MF
      Am. J. Respir. Crit. Care Med., 2008;177(11):1233-41.
      Species: Porcine
      Sample Type: BALF
    12. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
      Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
      Eur. Respir. J., 2007;30(6):1167-72.
      Species: Porcine
      Sample Type: Serum
    13. Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
      Authors: Moller S, Lauridsen C
      Cytokine, 2006;35(1):6-12.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    14. Fibrinogen induces chemotactic activity in endothelial cells.
      Authors: Seeger FH, Blessing E, Gu L, Bornhold R, Denger S, Kreuzer J
      Acta Physiol. Scand., 2002;176(2):109-15.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    15. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
      Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
      Mol. Ther., 212;20(6):1204-11.
      Species: Porcine
      Sample Type: Plasma
    Expand to show all
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Porcine CXCL8/IL-8 Quantikine ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed


    Customer Information