Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
What are ProDots® Proteins?
ProDots® proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots® proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL.
E. coli-derived human IGF-I protein Gly49 - Ala118
Reconstitution For a stock solution, reconstitute at 200 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
1 μg/lane of ProDots® Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 7 kDa.
Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.
The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
Have you used ProDots Recombinant Human IGF-I Protein?
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