Detection of beta 2‑Microglobulin in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with mouse anti-rat CD3 phycoerythrin‑conjugated monoclonal antibody and either (A) Mouse Anti-Rat beta 2‑Microglobulin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7438G) or (B) Normal Mouse IgM Alexa Fluor 488 Control (Catalog # IC015G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta 2-Microglobulin
beta 2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.
Entrez Gene IDs:
567 (Human); 12010 (Mouse); 24223 (Rat)
B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
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