Rat beta 2-Microglobulin Alexa Fluor® 488-conjugated Antibody

FAB7438G has been discontinued. View all beta 2-Microglobulin products.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Rat
  • Specificity
    Detects rat beta 2-Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 2-Macroglobulin is observed.
  • Source
    Monoclonal Mouse IgM Clone # 747502
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat beta 2-Microglobulin
    Ile21-Met119
    Accession # P07151
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of beta 2‑Microglobulin in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with mouse anti-rat CD3 phycoerythrin‑conjugated monoclonal antibody and either (A) Mouse Anti-Rat beta 2‑Microglobulin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7438G) or (B) Normal Mouse IgM Alexa Fluor 488 Control (Catalog # IC015G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta 2-Microglobulin

beta 2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat  beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

  • Entrez Gene IDs:
    567 (Human); 12010 (Mouse); 24223 (Rat)
  • Alternate Names:
    B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Rat beta 2-Microglobulin Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee