|Detection of beta 2‑Microglobulin in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with mouse anti-rat CD3 phycoerythrin‑conjugated monoclonal antibody and either (A) Mouse Anti-Rat beta 2‑Microglobulin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7438G) or (B) Normal Mouse IgM Alexa Fluor 488 Control (Catalog # IC015G). View our protocol for Staining Membrane-associated Proteins.|
beta 2-Microglobulin ( beta 2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta 2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta 2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat beta 2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.