Rat CD34 Antibody

Catalog # Availability Size / Price Qty
AF4117
AF4117-SP

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CD34 in Rat Liver.
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Product Details
Citations (18)
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Reviews (1)

Rat CD34 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CD34 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant porcine CD34 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat CD34
Glu37-Ile293
Accession # NP_001100672
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat CD34
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CD34 antibody in Rat Liver by Immunohistochemistry (IHC-Fr). View Larger

CD34 in Rat Liver. CD34 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Rat CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4117) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

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Citations for Rat CD34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Tankyrase Inhibition Attenuates Cardiac Dilatation and Dysfunction in Ischemic Heart Failure
    Authors: H Wang, H Segersvärd, J Siren, S Perttunen, K Immonen, R Kosonen, YC Chen, J Tolva, M Laivuori, MI Mäyränpää, PT Kovanen, J Sinisalo, M Laine, I Tikkanen, P Lakkisto
    International Journal of Molecular Sciences, 2022-09-02;23(17):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Chronic Intermittent Hypoxia Increases Cell Proliferation in Hepatocellular Carcinoma
    Authors: L Carreres, M Mercey-Res, K Kurma, J Ghelfi, C Fournier, O Manches, F Chuffart, S Rousseaux, M Minoves, T Decaens, H Lerat, Z Macek Jilk
    Cells, 2022-06-28;11(13):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. DEN-Induced Rat Model Reproduces Key Features of Human Hepatocellular Carcinoma
    Authors: K Kurma, O Manches, F Chuffart, N Sturm, K Gharzeddin, J Zhang, M Mercey-Res, S Rousseaux, A Millet, H Lerat, PN Marche, Z Macek Jilk, T Decaens
    Cancers, 2021-10-04;13(19):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Multilayered Human Skeletal Muscle Myoblast Sheets Promote the Healing Process After Colonic Anastomosis in Rats
    Authors: T Nakamura, U Yokoyama, T Kanaya, T Ueno, T Yoda, A Ishibe, Y Hidaka, M Umemura, T Takayama, M Kaneko, S Miyagawa, Y Sawa, I Endo, Y Ishikawa
    Cell Transplantation, 2021-01-01;30(0):9636897211009.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
    Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
    Cancer Cell Int, 2020-10-02;20(0):476.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Highly blood perfused, highly metabolically active pancreatic islets may be more susceptible for immune attack
    Authors: S Ullsten, D Espes, M Quach, M Fex, M Sandberg, PO Carlsson
    Physiol Rep, 2020-07-01;8(13):e14444.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Fibroblasts Colonizing Nerve Conduits Express High Levels of Soluble Neuregulin1, a Factor Promoting Schwann Cell Dedifferentiation
    Authors: BE Fornasari, M El Soury, G Nato, A Fucini, G Carta, G Ronchi, A Crosio, I Perroteau, S Geuna, S Raimondo, G Gambarotta
    Cells, 2020-06-01;9(6):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  8. Participation of Somatic Stem Cells, Labeled by a Unique Antibody (A3) Recognizing both N-glycan and Peptide, to Hair Follicle Cycle and Cutaneous Wound Healing in Rats
    Authors: C Katou-Ichi, H Nishina, M Tanaka, S Takenaka, T Izawa, M Kuwamura, J Yamate
    Int J Mol Sci, 2020-05-27;21(11):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  9. Complete regeneration of large bone defects in rats with commercially available fibrin loaded with BMP-2
    Authors: M Koolen, A Longoni, J van der St, O Van der Ja, D Gawlitta, H Weinans
    Eur Cell Mater, 2019-09-17;38(0):94-105.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Extension of Tissue Plasminogen Activator Treatment Window by Granulocyte-Colony Stimulating Factor in a Thromboembolic Rat Model of Stroke
    Authors: IC Dela Peña, S Yang, G Shen, H Fang Liang, S Solak, CV Borlongan
    Int J Mol Sci, 2018-05-31;19(6):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Cold water swimming pretreatment reduces cognitive deficits in a rat model of traumatic brain injury
    Authors: ZW Zhou, YD Li, WW Gao, JL Chen, SY Yue, JN Zhang
    Neural Regen Res, 2017-08-01;12(8):1322-1328.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Rat umbilical cord blood cells attenuate hypoxic-ischemic brain injury in neonatal rats
    Authors: K Nakanishi, Y Sato, Y Mizutani, M Ito, A Hirakawa, Y Higashi
    Sci Rep, 2017-03-10;7(0):44111.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  13. Responses of adventitial CD34(+) vascular wall-resident stem/progenitor cells and medial smooth muscle cells to carotid injury in rats
    Authors: Jian Song
    Exp. Mol. Pathol., 2016-11-14;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  14. Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy.
    Authors: Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S
    PLoS ONE, 2015-07-08;10(7):e0128910.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. A metastatic hepatoma model of rats using the 13762-MAT-B-III cell line: basic characteristics and potential as a tool for interventional oncology experiments.
    Authors: Choi J, Kim H, Baek S, Ryu Y, Chung J
    Anticancer Res, 2015-03-01;35(3):1333-8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Renal telocytes contribute to the repair of ischemically injured renal tubules.
    Authors: Li L, Lin M, Li L, Wang R, Zhang C, Qi G, Xu M, Rong R, Zhu T
    J Cell Mol Med, 2014-04-24;18(6):1144-56.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC
  17. Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing antibody: a possible marker of hair follicle development.
    Authors: Ichikawa C, Izawa T, Juniantito V, Tanaka M, Hori M, Tanaka K, Takenaka S, Kuwamura M, Yamate J
    Histol Histopathol, 2013-02-01;28(2):257-68.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. In vivo adipogenesis in rats measured by cell kinetics in adipocytes and plastic-adherent stroma-vascular cells in response to high-fat diet and thiazolidinedione.
    Authors: Tchoukalova YD, Fitch M, Rogers PM, Covington JD, Henagan TM, Ye J, Hellerstein MK, Ravussin E
    Diabetes, 2011-11-28;61(1):137-44.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Rat CD34 Antibody
By Anonymous on 05/15/2018
Application: IHC Sample Tested: Eye lens tissue Species: Rabbit