Rat CNTF Antibody

(3 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CNTF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human CNTF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant rat CNTF
    Ala2-Met200
    Accession # P20294.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat CNTF (Catalog # 557-NT)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of rat spinal cord
  • Neutralization
    Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 4-8 µg/mL in the presence of 200 ng/mL Recombinant Rat CNTF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. Recom­binant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND50 is typ­ically 4-8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTF
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.
  • Long Name:
    Ciliary Neurotrophic Factor
  • Entrez Gene IDs:
    1270 (Human); 25707 (Rat)
  • Alternate Names:
    ciliary neurotrophic factor; CNTF; HCNTF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus.
    Authors: Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A
    Front Neurosci, 2013;7(0):263.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells.
    Authors: Leaver SG, Cui Q, Plant GW, Arulpragasam A, Hisheh S, Verhaagen J, Harvey AR
    Gene Ther., 2006;13(18):1328-41.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
    Authors: Gard AL, Gavin E, Solodushko V, Pennica D
    Neuroscience, 2004;127(1):43-52.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
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