Rat gp130 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects rat gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human or recombinant mouse gp130 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 745314
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat gp130
    Gln23-Glu618 (predicted)
    Accession # P40190
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of gp130 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with PE-conjugated anti-rat CD3 antibody and either (A) Mouse Anti-Rat gp130 APC‑conjugated Monoclonal Antibody (Catalog # FAB5029A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: gp130

Glycoprotein 130 (gp130), also known as IL-6 signal transducer, IL-6 receptor beta, and oncostatin-M alpha subunit, is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of  IL-6 family member signals across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by a cytokine receptor homology region (CHR) that is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Over aa 23-618, rat gp130 shares 86% and 75% aa sequence identity with mouse and human gp130, respectively. Gp130 serves as a signal transducing receptor subunit for IL-6-type cytokines consisting of IL‑6, IL-11, Leukemia Inhibitory Factor (LIF), Oncostatin M (OSM), Ciliary Neurotrophic Factor (CNTF), IL-27, IL-35, Cardiotrophin-1 (CT-1), Cardiotrophin-2 (CT-2), and Cardiotrophin Like Cytokine (CLC) (2-7). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

  • References:
    1. Wang, Y. et al. (1992) Genomics 14:666.
    2. Muller-Newen, G. (2003) Sci. STKE pe40.
    3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
    4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
    5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
    6. Garbers,C. et al. (2015) Curr. Opin. Immunol. 34:75.
    7. Collison, L.W. et al. (2012) Nat. Immunol. 13:290.
  • Long Name:
    Glycoprotein 130
  • Entrez Gene IDs:
    3572 (Human); 16195 (Mouse); 25205 (Rat)
  • Alternate Names:
    CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; GP130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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