|Detection of gp130 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with PE-conjugated anti-rat CD3 antibody and either (A) Mouse Anti-Rat gp130 APC‑conjugated Monoclonal Antibody (Catalog # FAB5029A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.|
Glycoprotein 130 (gp130), also known as IL-6 signal transducer, IL-6 receptor beta, and oncostatin-M alpha subunit, is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of IL-6 family member signals across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by a cytokine receptor homology region (CHR) that is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Over aa 23-618, rat gp130 shares 86% and 75% aa sequence identity with mouse and human gp130, respectively. Gp130 serves as a signal transducing receptor subunit for IL-6-type cytokines consisting of IL‑6, IL-11, Leukemia Inhibitory Factor (LIF), Oncostatin M (OSM), Ciliary Neurotrophic Factor (CNTF), IL-27, IL-35, Cardiotrophin-1 (CT-1), Cardiotrophin-2 (CT-2), and Cardiotrophin Like Cytokine (CLC) (2-7). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).