Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED50 for this effect is typically 15‑75 ng/mL in the presence of 20 ng/mL Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL).
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Cynomolgus Monkey DcR3/TNFRSF6B
    (Ala30-His300)
    Accession # EHH65162
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala30
  • Predicted Molecular Mass
    56 kDa
  • SDS-PAGE
    58-67 kDa, reducing conditions
9297-DC
 
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Cynomolgus Monkey DcR3/TNFRSF6B Fc Chimera (Catalog #
9297-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is
15-75 ng/mL.
Background: DcR3/TNFRSF6B
DcR3 (Decoy Receptor 3), also known as TR6 and TNFRSF6B is a 35-40 kDa secreted member of the TNF Receptor superfamily (1). Mature cynomolgus DcR3 contains four tandem TNFR cysteine-rich domains and shares 92% amino acid sequence identity with human DcR3. It binds to the TNF superfamily ligands Fas Ligand, TL1A, and LIGHT (2-5) and interferes with their respective interactions with Fas, DR3, or HVEM and Lymphotoxin beta R (2-4). It blocks apoptosis triggered through either Fas Ligand, TL1A, or LIGHT (2, 3, 5, 6). DcR3 is up-regulated in a variety of cancers and enhances tumor cell immune evasion (2, 3, 7). It also promotes immune suppression by inducing dendritic cell apoptosis (8), inhibiting NK cell and CD8+ T cell activity (2, 4), and inhibiting the production of inflammatory cytokines during viral infection or autoimmunity (9, 10). In humans, proteolytic removal of the C-terminal 53 amino acids generates a shortened DcR3 that retains the ability to block LIGHT but not Fas Ligand induced apoptosis (11). DcR3 can also induce osteoclast formation from monocytes (12).
  • References:
    1. Siakavellas, S.I. and G. Bamias (2015) Inflamm. Bowel Dis. 21:2441.
    2. Pitti, R.M. et al. (1998) Nature 396:699.
    3. Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733.
    4. Zhang, J. et al. (2001) J. Clin. Invest. 107:1459.
    5. Migone, T.S. et al. (2002) Immunity 16:479.
    6. Han, B. and J. Wu (2009) J. Immunol. 183:8157.
    7. Ge, Z. et al. (2011) Exp. Ther. Med. 2:167.
    8. You, R.I. et al. (2008) Blood 111:1480.
    9. Huang, M.T. et al. (2015) J. Mol. Med. (Berlin) 93:1131.
    10. Mueller, A.M. et al. (2009) J. Neuroimmunol. 209:57.
    11. Wroblewski, V.J. et al. (2003) Biochem. Pharmacol. 65:657.
    12. Yang, C.R. et al. (2004) Cell Death Differ. 11 Suppl 1:S97.
  • Long Name:
    Decoy Receptor 3
  • Entrez Gene IDs:
    8771 (Human); 107126607 (Cynomolgus Monkey)
  • Alternate Names:
    DcR3/TNFRSF6B; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy
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